Overview
Workplace drug testing analyzes a specimen for the presence of specified substances. A collection site gathers the specimen, a laboratory or a rapid test analyzes it, and a physician reviews any non-negative result before it reaches the employer.
It differs from every other component described on this site in a basic way. The others retrieve records that already exist. A drug test creates new information at the moment it is administered, which is why it has its own collection procedures, its own chain of custody, and its own review step.
Testing splits into 2 worlds that follow different rules. Testing required by federal transportation regulation follows a prescribed federal procedure. Everything else is governed by the employer’s own policy within the limits state law sets.
Panels and What They Detect
A panel is the set of substances a test looks for, and the number in its name is the count of substance groups covered.
| 5 panel | The most widely used, and the panel the Department of Transportation requires for regulated testing. Covers amphetamines, cocaine, opiates, phencyclidine, and marijuana. The DOT version includes specified synthetic opioids within the opiate group. |
| 7 panel | Adds substances such as barbiturates and benzodiazepines. Common where an employer wants coverage beyond the standard 5 without a full expansion. |
| 10 panel | The most common expanded panel in non-regulated testing. Typically adds barbiturates, benzodiazepines, methadone, propoxyphene, and methaqualone. |
| 12 panel and above | Adds further substances, frequently prescription opioids and other medications of concern in a particular setting. |
Exact contents vary between providers. Two tests sold as 10 panel can cover slightly different substances, so the panel number describes scope rather than a fixed list.
Two points about what a panel means:
- A test finds only what it looks for. A substance outside the panel is not detected, however recently it was used.
- Detection is not impairment. A test identifies a substance or its metabolite in a specimen. It does not establish that the person was impaired at the time of the test, and for most substances the detection window extends well beyond the period of any effect.
Alcohol is usually handled separately, through breath or saliva testing, and is measured as a concentration rather than a presence.
Specimen Types
| Urine | The most common specimen. Detects recent use, with windows varying by substance from about 2 to 4 days for many drugs and longer for chronic marijuana use. |
| Oral fluid (swab) | Collected by swab from inside the mouth, under direct observation, which removes most opportunities for substitution. Detects more recent use than urine, over a shorter window. |
| Hair | Detects a longer history, commonly cited at around 90 days, and does not detect very recent use because of the time hair takes to grow. |
| Blood | Used less often in employment. Reflects current presence rather than history, so it is the closest to a measure of recent use. |
| Breath | Used for alcohol, and measures current concentration. |
Each specimen answers a slightly different question, which is why a hair test and a urine test on the same person can disagree without either being wrong.
How a Result Is Produced
A properly run test has more steps than most people expect, and the steps exist to prevent an incorrect positive.
- Collection under chain of custody. The specimen is documented from collection through analysis, with each transfer recorded, so the sample tested can be traced to the person who provided it.
- Initial screening test. A rapid, inexpensive test that flags specimens for further analysis. A screening test alone is not a result.
- Confirmation testing. Any specimen flagged by the screen is analyzed again by a more precise method, which identifies the specific substance rather than a class of substances.
- Review by a physician. A non-negative confirmed result goes to a medical review officer before the employer sees anything.
A cutoff level applies at each analytical stage. A specimen containing a substance below the cutoff is reported as negative, which is deliberate. Cutoffs are set to avoid reporting trace amounts and incidental exposure.
Rapid Testing
A rapid test, also called an instant or point of collection test, performs the screening step at the collection site rather than at a laboratory. A device produces a preliminary reading within minutes.
A negative rapid result is generally reported as negative and the process ends there, which is why rapid testing is used where speed matters. A non-negative rapid result is not a positive. The specimen goes to a laboratory for confirmation testing and medical review exactly as it would otherwise, and only that outcome is reported.
The tradeoff is that rapid testing shortens the timeline for the majority of tests, which return negative, while a non-negative result takes the same time it always would. Rapid testing is not permitted in place of the prescribed laboratory process for DOT regulated urine testing.
The Medical Review Officer
A medical review officer, usually called an MRO, is a licensed physician trained to interpret drug test results. The role exists because a laboratory can establish what is in a specimen and cannot establish why.
When a confirmed non-negative result arrives, the MRO contacts the individual privately and gives them an opportunity to provide a legitimate medical explanation, such as a valid prescription. If the explanation accounts for the finding, the MRO reports the result to the employer as negative. The employer is not told the substance or the reason.
This step is why an employer generally receives a conclusion rather than a laboratory report, and it is a meaningful privacy protection. A prescription an applicant discloses to the MRO does not travel to the employer.
Results Other Than Positive or Negative
- Dilute. The specimen is unusually watery, which can result from drinking large amounts of fluid. It is not a positive, and a retest is the usual response.
- Adulterated. A substance was added to the specimen to interfere with testing.
- Substituted. The specimen is not consistent with human urine.
- Invalid. The laboratory could not produce a valid result.
- Refusal. Failing to appear, leaving before completion, or declining to provide a specimen. Under regulated testing a refusal is generally treated the same as a positive.
- Insufficient specimen. Not enough was provided. Procedures allow a defined period to try again, and a medical evaluation where the shortfall persists.
- Lost in transit. The specimen did not reach the laboratory, or arrived in a condition that prevented testing. It is a handling failure rather than a result, and the response is a new collection. The individual is not responsible for it, and it should not be recorded as a refusal.
- Cancelled. The test cannot be reported as either positive or negative, usually because of a collection or documentation problem. A recollection generally follows.
Alcohol Testing
Alcohol is tested separately from drugs and behaves differently in nearly every respect.
It is measured as a concentration rather than a presence. A drug test reports whether a substance is there. An alcohol test reports how much, expressed as a breath alcohol concentration, and the result is compared against a threshold.
Because alcohol leaves the body within hours, a test reflects a current or very recent state rather than a history of use. That makes it a far better indicator of present impairment than a drug test, and a far worse indicator of anything else.
Breath testing is the usual method, conducted on site by a trained technician using an approved device. Saliva screening is also used. Under DOT rules, a screening result at or above the regulatory threshold is followed by a confirmation test on an evidential breath testing device after a short waiting period.
Alcohol testing is treated as a medical examination under federal disability law. Drug testing is not. That difference determines when each may occur. A pre-offer drug test is generally permitted, while alcohol testing sits after a conditional offer along with other medical examinations. See our Physical Exams page.
Alcohol is also lawful for adults, which changes the analysis. Employers generally test for it in connection with being at work rather than for use itself, so alcohol testing is concentrated in reasonable suspicion, post-accident, and random testing rather than at hire.
When Testing Happens
Employers test at several points, and the trigger shapes what the test is for.
- Pre-employment, most commonly after a conditional offer.
- Random, using a selection method that gives each covered person an equal chance, which is what distinguishes it from testing chosen by a manager.
- Reasonable suspicion, based on specific observed behavior, usually documented by a trained supervisor.
- Post-accident, under criteria the employer or the regulation sets.
- Return to duty and follow up, after a previous violation, as part of a defined process.
State law affects several of these. Some states restrict random testing, some place conditions on post-accident testing, and some require specific notice or policy provisions before any testing occurs. Our State Laws Overview covers how the states differ and links to the current position for each one.
DOT and Non-DOT Testing
Testing required by the U.S. Department of Transportation, usually called DOT testing, follows a prescribed federal procedure. It sets which substances are tested, which laboratories may be used, what the cutoff levels are, how the collection is conducted, what form is used, and how results are reviewed and reported. The employer has almost no discretion over any of it.
DOT testing applies to employees in safety sensitive roles across the transportation agencies, covering commercial drivers, aviation, rail, transit, pipeline, and maritime work. Each agency applies the same underlying testing procedures to its own population.
For commercial drivers, violations are also reported to a federal database, covered on our FMCSA Clearinghouse page.
Non-DOT testing is the employer’s own program. The panel, the specimen type, the triggers, and the consequences are set by policy, subject to whatever state law requires. This is why 2 employers can test very differently and both be compliant.
An employer may run both. A trucking company tests its drivers under DOT rules and its office staff under its own policy, and the 2 programs are kept separate because the procedures differ.
Why a Drug Test Is Treated Differently
A test for illegal drug use is not treated as a medical examination under federal disability law. That is why it can be required before a job offer, while a physical examination cannot. Alcohol testing does not share that treatment and is handled as a medical examination.
The distinction has practical consequences. It is the reason a pre-employment drug test is ordinarily lawful at a stage when most other health related inquiries are not, and the reason alcohol testing is generally positioned later in the hiring process.
Testing can also collect information beyond illegal drug use, since a result may reveal a lawfully prescribed medication. The medical review officer step is part of how that information is kept away from the employer.
Marijuana
Marijuana is the most complicated substance in workplace testing, and the complication is legal rather than analytical.
It remains a controlled substance under federal law, so it stays on federally regulated panels regardless of state law. At the same time, many states have legalized it in some form, and a number have enacted employment protections that limit what an employer may do about off duty use or about a positive test alone.
There is also a technical dimension. Marijuana metabolites can remain detectable long after any effect has passed, particularly with frequent use, so a positive result is a weaker indicator of impairment than for most other substances. Several state laws are built around exactly that point.
Because protections vary substantially and continue to change, the position depends entirely on the state where the work is performed. Our State Laws Overview carries a marijuana and drug testing section for each state.
Where It Fits Under the FCRA
Where a screening company arranges testing and reports the result to an employer, that result forms part of a consumer report. Written disclosure and authorization are required, accuracy obligations attach to what is reported, and the individual has the right to dispute an incorrect result. Our FCRA Overview covers those requirements in full.
Where an employer collects and receives a test result directly, without a third party in the chain, the consumer reporting rules may not attach in the same way. Other obligations still apply, including state testing laws and disability law.
Where a test result contributes to a decision not to hire, the required notice steps apply. This matters here because the individual may need to raise a laboratory error, a chain of custody problem, or a medical explanation. See The Adverse Action Process.